Evaluation of Protective effect of reduced Glutathione on 5-Fluorouracil-induced changes in Cholesterol Profile

The study was designed with an aim to evaluate the protective effects of reduced glutathione on 5fluorouracil (5-FU)-induced Cholesterol profile in vitro. Goat blood was used as lipid source for the model. In the cholesterol profile total cholesterol (TC) and high density lipoprotein (HDL) cholesterol content of goat blood was determined. 5-FU and reduced glutathione were added at a concentration of 0.012, 0.05 mg / g of goat blood respectively. The study reveals that 5-FU has the induction capacity to produce changes in cholesterol profile (12.164 and 13.328% for total cholesterol as well as -5.502 and -16.594 for HDL cholesterol at 1 and 2 hrs of incubation with respect to corresponding controls). But after addition of reduced glutathione with 5-FU total cholesterol level was decreased (7.724 and 8.448%) and HDL cholesterol level was increased (29.312 and 12.426%) in comparison to 5-FU treated group. Incubation of blood samples only with reduced glutathione also shows a tendency of decrease in total cholesterol (-16.888 and -16.852%), but HDL-cholesterol contents (27.016 and 4.914%) were increased in comparison to control or 5-FU-treated group respectively. These observed data indicate that reduced glutathione has protective effect on 5-FU-induced changes in cholesterol content. Interpretation of the results is supported by analysis of variance and also by statistical multiple comparison analysis using least significant different procedure. For total cholesterol content reduced glutathione treated group is statistically significantly different from 5-FU treated as well as 5-FU and reduced glutathione-treated group. In case of HDL-cholesterol content 5FU treated group is statistically significantly different from 5-FU and reduced glutathione-treated group as well as only reduced glutathione-treated group.